Previous investigators have shown that the frequency compression is related to the muscle fatigue and the decreasing conduction velocity of muscle fibers. The aim of the present study was to investigate the relationship between endurance times and frequency parameters such as mean power frequency and median frequency in the surface EMG signal during isotonic contractions. Eight healthy subjects performed voluntary isotonic contractions of biceps Brachii muscle until their endurance times which were determined when the subject could no longer follow the contraction cycle. The regressive slopes of mean power frequency and median frequency were used to describe the frequency compression of the surface EMG signal, and to test the predictability of endurance time. As results of experiment, significant correlations were found between endurance time and the regressive slopes of mean power frequency and mean frequency computed over 50%Tend of endurance time.