The objective of this study was to investigate the comparison of transferable embryos and pregnancy rate between Hanwoo and Chickso. The results obtained were as follows: No differences were observed in the efficiency of superovulation rates on Hanwoo 78%, and Chickso 85%, respectively. The mean number of total embryos are each $14.76{pm}2.16$ and $6.23{pm}1.07$. So the mean number of transferable embryos are each $10.94{pm}1.91$ and $4.58{pm}1.05$. In addition, the mean number of total Hanwoo embryo from <10 and $10{leq}$ of corpora luteum was $0.50{pm}0.50$, $11.56{pm}1.92$, respectively. In case of Chickso, The mean number of transferable embryo from <10 and $10{leq}$ of CL was $2.75{pm}1.39$, $6.00{pm}1.00$, respectively. The pregnancy rates were Hanwoo 40%, and Chickso 37% following transfer of fresh embryos produced in vivo. Also, the pregnancy rates of Chickso 60% were significantly greater (p<0.05) than the Hanwoo 42.48% following transfer of following transfer of frozen embryos, respectively. In conclusion, these results suggest that Chickso may be effectively used for transferable embryos production in Hanwoo. Although the transferable embryos number was not enough, it seems the Chickso greatly affect pregnancy rate. The results indicated that the possibility of transferable embryos from Chickso for embryo transfer could be confirmed in this study. Based on the present findings, it was suggested that it is very important to evaluate in vivo embryo production and pregnancy rate of embryo transfer following superovulation for effective Hanwoo and Chickso production.