For development of dyeability, the cotton fabric was dyed repeatedly with persimmon juice by padding mangle. We evaluated the mechanical properties and hand value by Kawabata Evaluation System, and observed the change of surface morphology. The results obtained from this study were as follows. With the increase of repeating padding times of dyeing, the linearity of load-extension curve and tensile energy per unit length of the cotton fabric were increased, but the tensile resilience of fabric was decreased. The value of shear stiffness and shear hysteresis were increased. Also compression resilience and linearity of compression thickness curve were increased. The cotton fabric dyed with persimmon juice had shown the thickness and weight increase as the number of padding increase. As repeating times of dyeing with persimmon juice were increased, among the 6 hand values, the item of stiffness, anti-drape stiffness, fullness and softness were increased, while flexibility with soft feeling and crispness were greatly decreased. The amount of coated persimmon juice on the surface of the fabric was gradually increased as the padding times of dyeing. And cotton fabrics were dyed evenly with persimmon juice by padding mangle.