A spiritual sense may be present in the human brain. Demonstration of the spiritual sense depends on measuring the behavioral effects of spiritual experiences. These experiences can be induced by prayer, specific chemicals and other stimuli that are known to interact with pyramidal neurons of layer 5 in the cerebral cortex. This work examines the hypothesis that pyramidal neurons are the seat of the spiritual sense. Healers use the spiritual sense to comfort and heal their patients, especially with prayer. Many doctors do not encourage prayer and could learn from healers to use the spiritual sense. Patients should be educated to use prayer in order to get the comfort they need for healing.