This study attempted to evaluate the direct and indirect effect of emotional labor, job and psychosocial stress, and fatigue of beauty industry workers on their musculoskeletal diseases. The findings of this study showed that, among the workers with perceived symptoms of musculoskeletal diseases, the body part with the most commonly occurring symptom was the shoulder, followed by neck, low back, wrist, arm, foot and knee. As for the effect of work-related psychological factors on musculoskeletal diseases, it was found that the workers with musculoskeletal diseases were more affected by emotional labor and fatigue than non-symptom workers. It was also shown that when their job stress increased, the fatigue level also increased, though their psychosocial stress decreased. In addition, it was found that both direct and indirect effects of emotional labor on fatigue were high, and the indirect effect of job stress through fatigue on musculoskeletal diseases was significant.