A layout of an LNG FPSO should be elaborately determined as compared with that of an onshore plant because many topside process systems are installed on the limited area; the deck of the LNG FPSO. Especially, the layout should be made as multi-deck, not single-deck and have a minimum area. In this study, a multi-floor layout for the liquefaction process, the dual mixed refrigerant(DMR) cycle, of LNG FPSO was determined by using the optimization technique. For this, an optimization problem for the multi-floor layout was mathematically formulated. The problem consists of 589 design variables representing the positions of topside process systems, 125 equality constraints and 2,315 inequality constraints representing limitations on the layout of them, and an objective function representing the total layout cost. To solve the problem, a hybrid optimization method that consists of the genetic algorithm(GA) and sequential quadratic programming(SQP) was used in this study. As a result, we can obtain a multi-floor layout for the liquefaction process of the LNG FPSO which satisfies all constraints related to limitations on the layout.