Purpose: The purpose of this study was to compare the quality of life(QOL) between middle-aged women and men. Method: In this study, we surveyed 482 middle-aged women and men living in Seoul aged between 40 and 64. Data were analyzed t-test, ${chi}^2$-test, and 2-way ANOVA. Results: There was no significant difference between satisfaction and importance of the life of middle-aged women and men of the overall. However, while the case of middle-aged women showed a significant difference in the emotional support from people who are not family members, middle-aged men showed significant differences between the importance and satisfaction of the life with spouse, the ability to take care of family responsibilities, sex life, energy(fatigue), personal appearance, and pain. In addition, the overall quality of life scores showed no gender difference between the middle-aged women and men but in the quality of life both genders, the family area of the sub-area showed high scores. Conclusion: It is suggested that further repetitive studies for middle-aged women and men are needed by using the same tool of this study and various health promotion programs based on the result of this study should be developed for middle-aged.