Objectives : The purpose of this study is to compare with the effect of general oriental medical treatment with or without ankle joint Chuna Manual Therapy for acute ankle sprain. Methods : We investigated 36cases of ankle sprain patients, and devided patients into two groups: One was treated general oriental medical treatment(Control Group) and the other was treated ankle joint Chuna Manual Therapy with general oriental medical treatment(Experimental Group). Results : 1. Each Group had significantly decrease in Visual Analogue Scale(VAS) and significantly increase in Ankle-Hind-foot Scale(AHS) after treatment. 2. Experimental Group was significant differences in Visual Analogue Scale(VAS) and Ankle-Hind-foot Scale(AHS) compare to Control Group. Conclusions : In this clinical study, general oriental medical treatment with ankle joint Chuna Manual Therapy was more effective in reduce ankle sprain pain and improve ankle joint function.