The knowledge of native wild mushrooms traditionally using in Jeju Island, that have specific native environment and various biodiversity, was investigated. From Jeju and Seogwipo cities including 7 provinces 4 locals, so total 50 villages, data was collected in the entire area and voice of the 93 elderly people between the age of fifty and ninety were recorded. As a result, total 23 native wild mushrooms were used in which 2 species were poisonous mushrooms, 7 species were medicinal mushrooms, 2 species was used for folk religion, 2 species were not used for living but for attention and 12 species were edible mushrooms. Also, a total of 267 cases of traditional knowledge was collected, in which 197 cases were about edible mushrooms for 12 species, 43 cases were about poisonous mushrooms for 2 species, 16 cases were about medicinal mushrooms for 7 species. Interestingly, the fortune for agriculture was told depending on spore mass release of Cyathus stercoreus which grows in animal feces and compost. It is considered as the distinctive traditional knowledge of Jeju Island.