In this paper, study the substantial issues which occurs upon free flight environment by performing separation assurance under multiple conflict(over 3 Aircraft), recovery en route under the terms of time constrains and fixed way point after the conflict avoidance, correlations between conflict detection distance and separation assurance by utilizing Autonomous flight algorithm. Result of this experiment demonstrates that the extension of detection distance is advantageous to solution of separation assurance and enhancing of flight efficiency, choose to maneuver by applying time constrain terms and fixed way point according to the situation of conflict prediction in case of recovery maneuver after the conflict avoidance. And separation assurance can be solved by applying 2 degrees or more of bank angle. When choosing the optimal bank angle could be drastically improved flight efficiency.