This study is the experimental study to improve the insulation of the fastening unit system, which has the most vulnerable insulation in the curtain walls. The Fastening Units were designed and fabricated to minimize the connection part of mullions. In addition, slight movements were taken into account and the performance of the middle layer was evaluated by forming an insulation layer with the vibration-proof rubber and the silicon to satisfy the mechanical and thermal performance criteria. A total of 10 experiments were performed under various conditions, such as indoor-outdoor temperature difference, type of insulation material, thickness of insulation material, and others. using the fabricated Fastening Units. As a result, the vibration-proof rubber insulation showed the temperature difference of $2.2^{circ}C-5.0^{circ}C$, and the silicon insulation showed the temperature difference of $2.8^{circ}C-4.5^{circ}C$, compared to the non-insulated Fasteniirature difference, typesng Units. When these results were compared with the psychometric chart graph, the insulated Fastening Unit designed in this study can be considered to prevent the dew condensation.