The purpose of the present study was to investigate the antioxidant and pharmacological properties of fruit, stem, and leaf extract of Psychotria nilgiriensis. Acetone extract of P. nilgiriensis fruit was found to have highest total phenolics (505.74 mg GAE/g extract), tannin (460.78 mg GAE/g extract), and flavonoid (67.78 mg RE/g extract) content. In vitro antioxidant studies revealed that the acetone extract of fruit posses significant antioxidant activity in DPPH radical scavenging, $ABTS^{{cdot}+}$, and ferric reducing antioxidant power (FRAP) assays. In vivo studies revealed that P. nilgiriensis fruit (400 mg/kg) showed good analgesic activity in hot plate method (72%), acetic acid induced writhing test (53%) and also showed significant in carrageenan induced inflammation (73.54%). GC/MS analysis of fruit showed the presence of sesquiterpenes. The study highlights the significant medicinal value of the plant.