Environment has become a main issue nowadays. People began to show big interest in "futuristic means of transportation", which is an efficient method in $CO_2$ emissions reduction and decreasing use of oil. Due to the noise and emissions of two-wheel vehicle of internal combustion engine, electric two-wheeled vehicles have been supplied in downtown. The electric two-wheeled vehicles use battery as power source. The performance of lithium-ion battery changes as the ambient temperature changes. In this paper, analysis of performance variance of electric two-wheeled vehicles influenced by the temperature using the chassis dynamometer and the environmental chamber was carried out.