The purpose of this study is to investigate the relationship between organizational culture(constructive and defensive culture) and emotional labor along with the social supports (supervisor support, colleague support). Using data obtained from a survey of 419 flight attendants of a major airline company in south Korea. The results are as follows: Organizational culture had a significant effect on emotional labor. At this point, colleague support mediated the relationship between constructive culture and emotional labor. In addition, supervisor support and colleague support had a mediating role between defensive culture and emotional labor. This result concludes that flight attendants positively accept the essential manuals and safety regulations in the special working condition with a unified team service. Furthermore, the results show that social supports are an important job resource for flight attendants helping them reach their work-related goals.