The flutter reliability analysis of long span bridges requires use of a software tool that predicts the uncertainty in a flutter response due to uncertainties in the model formulation and input parameters. Existing flutter analysis numerical codes are not capable of dealing with stochastic uncertainty in the analysis of long span bridges. The goal of the present work is to develop a software tool (FREASB) to enable designers to efficiently and accurately conduct flutter reliability analysis of long span bridges. The FREASB interfaces an open-source Matlab toolbox for structural reliability analysis (FERUM) with a typical deterministic flutter analysis code. The paper presents a brief introduction to the generalized first-order reliability method implemented in FREASB and key steps involved in coupling it with a typical deterministic flutter analysis code. A numerical example concerning flutter reliability analysis of a long span suspension bridge with a main span of 1385 m is presented to demonstrate the application and effectiveness of the methodology and the software.