Objectives : The purpose of this study was to identify the factors that affect working environment related musculoskeletal subjective symptoms among dental hygienists. Methods : The subjects in this study were 232 dental hygienists working at dental clinics and general hospitals in Daegu. The data has collected through the self-questionnaire survey from July 9th 2012 to July 31th. Results : 1. Musculoskeletal subjective symptoms were 85.3% in shoulder(right), 81.9% in neck, 74.6% in shoulder(left), 65.5% in wrist(right), 56.5% in lower leg(right). 2. Pain frequency of musculoskeletal subjective symptoms were the highest 24.7% in neck. Seeing the severity pain was the highest 9.0% in foot(left). The investigation of work interference related to substantially pain showed the highest 18.5% in wrist(right). 3. Musculoskeletal subjective symptoms correlated with general characteristics such as age, marital status, regular exercise and medical check-up(p<.05). 4. Musculoskeletal subjective symptoms correlated with working environments such as working career, the night treatment, the average daily number of patients handled, the average daily standing work hours, the regular rest, the major job in work place and physical burden(p<.05). Conclusions : The education or program on wrong working habits and bad postures of dental hygienists is needed to prevent musculoskeletal disorder.