"Drop-in" tests of R-1234yf in a mobile air conditioning (MAC) system were conducted, and the system performance results were compared with those of the same system using R-134a. The performance tests were performed in a psychrometric calorimeter test facility based on enthalpy-difference method. The system performance was investigated under low-, middle- and high-load conditions. The results showed that the optimum refrigerant charge of R-1234yf was approximately 90% compared with that of R-134a under the same MAC system. When operated under optimum refrigerant charge, the system cooling capacity and COP of R-1234yf was 11% and 8.3% lower than that of the R-134a system, respectively. When wind tunnel test was conducted on a practical vehicle, the result showed that the carriage temperature in the vehicle with the R-1234yf system dropped slower. The average carriage temperature and blower outlet temperature were $2^{circ}C$ higher than those of the R-134a system during the test.