In spite of the ecological value of the DMZ, there have not been established guidelines for sustainable conservation and use on the DMZ and adjacent area. In this study we suggested a method about zoning the conservation and utilization area considering environmental data and the characteristic of the DMZ region, and we make the guideline for conservation of the DMZ region based on the zone. Using 10 legal protection areas, the Environmental Conservation Value Assessment Map (ECVAM) and the boundaries of the DMZ region, we evaluated this region into conservation areas, management areas, and utilization areas. The results showed that as much as 91.8% of the DMZ region was evaluated as conservation areas, 6.9% as management areas, and 1.3% as utilization areas. After consultation with experts we suggested management principles, the direction, and the guideline based on the planning process considering the evaluation map. The study suggests principles and guidelines for management, and the guidelines could be a effective tool for the sustainable conservation and management strategy of DMZ region.