우리나라 고유 담수어류 참종개 $Iksookimia$ $koreensis$의 난발생 및 초기생활사를 연구하기 위하여 2010년 7월 강원도 횡성군 서원면 옥계리에서 족대를 이용하여 친어를 채집하여 실험을 실시하였다. 채집된 성숙한 친어는 복강에 Ovaprim을 주사하여 12시간 경과 후 복부압박법으로 채란하여 건식법으로 인공수정시켰다. 산란된 성숙란은 약간의 점착성을 띤 엷은 노란색의 분리침성난이었으며 난경은 $1.40{pm}0.04mm$였다. 수온 $23^{circ}C$에서 수정 후 50시간 후에 부화하였으며, 크기는 전장 $4.7{pm}0.21mm$였다. 부화 후 5일경에 난황이 모두 흡수되었으며 전장 $7.1{pm}0.25mm$로 성장하였다. 부화 후 17일 후에 지느러미 기조가 모두 정수로 되어 치어기로 넘어갔으며 전장 $12.2{pm}1.10mm$로 성장하였다. 부화 후 80일에는 성어와 유사한 체측반문 형태를 보였으며 전장 $31.0{pm}3.98mm$로 성장하였다.
In the present study, egg development and early life history of Korean spined loach, $Iksookimia$ $koreensis$, were observed. Adult fish were sampled using spoon nets in Okgye-ri, Hoengseong-gun, Gangwon-do, Korea in July 2010. Eggs were obtained after injecting females with Ovarprim. Eggs were then artificially fertilized in the laboratory using the dry method. Mature eggs were slightly adhesive and transparent with a light yellowish color, and measured $1.40{pm}0.04mm$ (mean${pm}$SD) in diameter. Hatching of the embryo occurred approximately 50 h after fertilization in the water at $23^{circ}C$, and newly hatched larvae were averaged $4.7{pm}0.21mm$ in total length. 5 days after hatching, the averaged total length of larvae was $7.1{pm}0.25mm$ and their yolk sacs had been completely absorbed. 17 days after hatching, fish started to enter the juvenile stage and reached $12.2{pm}1.10mm$ in total length. 80 days after hatching, the band patterns and external form of juvenile fish were similar to those of adults, and they averaged $31.0{pm}3.98mm$ in total length.
