Objectives: The purpose of this study was to evaluate work-related risk factors of musculoskeletal disorders (MSDs) in various departments and tasks at an oriental medicine hospital. Methods: 269 hospital workers were selected for the study. The questionnaire survey included general characteristics, work-related characteristics and MSDs. Cross-tabulation analysis, multiple logistic regression, adjusted for age, work time, job demand and workload, were used to evaluate the effects of work-related factors on MSDs. Results: There were differences in MSDs depending on gender, age, occupation, and work-related factors. Prevalence rates of MSDs were 23.79% shoulder, 14.13% back, 10.41% neck. Risk factors for MSDs was packaging of drugs, transfer, and exercise therapy of physical therapist. Shoulder pain was significantly influenced by using power tools, over-reaching, and job demand while back pain was significantly influenced by weight-lifting operations and keyboard typing. Conclusions: The results of this study suggest that consideration of MSDs characteristics of workers in oriental medicine hospital is necessary to change unsuitable work environments and educate on preventing MSDs.