We evaluated Al-tolerance in 44 interspecific lines (32 upland and 12 lowland) developed from the crosses of $Oryza$ $sativa$ and $O.$ $glaberrima$ called New Rice for Africa (NERICA) with 2 $O.$ $glaberrima$ lines and 13 $O.$ $sativa$ varieties under hydroponic culture containing 0.15, 0.3, 0.6, and 1.2 mM Al (+Al) and 0 mM Al (-Al as a control). Ten upland and four lowland NERICA lines showed strong Al-tolerance judging from their higher relative root and shoot dry weights (percentage ratios of dry weights in the Al treatments to the control) than those of the tolerant $O.$ $sativa$ check of IR 53650. Their tolerance was supported by relatively higher root Al accumulation (dark blue color) opposite performance with common knowledge (shown pale blue color) in root using hematoxylin staining compared to the Al-susceptible genotypes identified based on relative root and shoot dry weights in the study. Net Al concentration was higher in roots than in shoots in all +Al conditions for all genotypes; however, a clear difference in the Al concentration among the Al-tolerant, Al-moderately tolerant, and Al-susceptible genotypes was observed in the shoots. Al concentrations in the shoots of the Al-tolerant and Al-moderately tolerant upland and lowland NERICA lines were significantly lower than those of its Al-susceptible counterparts in the groups under 0.6 and 1.2 mM Al conditions, respectively. Differences in root and shoot growth among the Al-tolerant, Al-moderately tolerant, and Al-susceptible NERICA lines were clearer under strong Al toxic conditions (0.6 and 1.2 mM Al) than under weak Al toxic conditions (0.15 and 0.3 mM Al).