Clothing is easily contaminated with various soils, including triglyceride-based sebum and sweat. These triglycerides generate malodors as a result of oxidation in the air and by hydrolysis caused by lipases from the microorganisms living on our skin. This research focused on the reactions of a skin flora, Staphylococcus epidermidis, with triolein on cotton fabric. The growth rate of S. epidermidis increased with increasing concentration of the nutrient medium. The growth rate of S. epidermidis on a triolein-soiled cotton swatch was higher than that on a control swatch. FT-IR analysis of extracts from triolein-soiled cotton fabric cultured with S. epidermidis showed changes in the molecular structure of triolein. When the incubation time was increased to above 72 h, the carboxylic acid C=O stretch increased continuously, indicating continuous degradation of triolein. GC-MS was used to identify the volatile components, i.e., the odor constituents. By 168 h of cultivation of S. epidermidis on triolein-soiled cotton fabric, the amount of aldehydes had greatly increased, and unsaturated aldehydes, alcohols, and a ketone were generated. These are formed by oxidation of triolein and by enzymatic hydrolysis of ester bonds. S. epidermidis and the volatile components were removed best in the bleach-added detergent solution. The effects of laundering solutions on the removal of microorganisms were more conspicuous than on the removal of volatile components.