Objectives : The study was carried out to find out the experience of musculoskeletal disorder symptoms of dental hygienists and evaluate the risk with ergonomic evaluation method(RULA). Methods : The subjects in this study were 344 dental hygienists working at dental clinics and general hospitals in Seoul, Gyeonggi, Daejeon and Chungnam/Chungbuk. The data were collected through the self-questionnaire survey from July 26th 2010 to September 30th 2010. Results : The results showed that those who working at dental hospital, network dental clinics, with 1-5 years of clinical experience, long working hour and sitting hour, no rest hour, more physical and mental burden had higher rate of the experience of musculoskeletal disorder symptoms compared with other groups. The final score of RULA evaluation results was 6 on average requiring continuous observation and urgent demand for work improvements. According to the result of RULA evaluation by work, the orthodontic clinics were 4.5, prosthodontic clinics were 5.0, and dental surgery clinics were 6.8 being the most risky. Conclusions : The study showed high complaints rate on musculoskeletal disorders in dental hygienists. Thus, various measures including provision of proper working and rest hours, use of ergonomic working equipments, strengthening the health education for desirable working posture and the development of musculoskeletal disorder prevention program should be needed.