This study mainly refers to the influence of micro-biologically induced mineral precipitation able to be used as innovative bio-nano technology on the mechanical performance of mortar. Newly selected microorganisms at Jeju Island and sporosarcina pasteurii remarked in the previous study were examined by applying their biomineralization to the mortar. Microorganism mixed mortar specimens were compared with each other and analysed. In this study, experimental groups, mortar specimens mixed with newly selected microorganims at the specific region, were superior control groups mixed with only 0.08% lime water in the compressive strength test and flexural strength test. At curing time passed, microorganism effected the increase of mortar strength and the rate of increase in the mortar compressive strength was decreased. This decrease is considered as reduction of biomineralization on which microorganism directly influenced because microorganism usually sporulates in poor environment like mortar environment. It was identified visually through SEM analysis that microorganism can make minerals. Mineral crystal and components were analysed through XRD and EDS analysis, finally it was demonstrated that biomineralization can improve mortar properties.