The purpose of this paper is to investigate the voice onset time (VOT) of the English voiceless stops [p, t, k] found in the Buckeye Corpus of Conversational Speech [1]. Three young female speakers were chosen for this study and their VOT values were semi-automatically extracted along with other factors. The factors used for the analysis were place of articulation, location in word, syllabic stress, content word or not, word frequency calculated from the corpus, and the speech rate expressed in syllables per second. Results showed that, for the three places of articulation of each speaker, all the factors had a statistically significant effect on the VOT values. This paper has significance in that the materials used for the analysis were from a corpus of spontaneous natural English speech.