Among lactic acid bacteria (LAB) isolated from mustard leaf kimchi, MLK27 was the most efficient strain in terms of adhesion to HT-29 cells. Competitive, exclusive, and displaceable inhibition of Listeria monocytogenes KCTC 3569 adhesion by LAB was strain-dependent. MLK27 strain displayed the highest adhesion inhibition against L. monocytogenes and produced organic acids, hydrogen peroxide, and bacteriocin. Cell-free culture supernatants (100 ${mu}L/mL$) and bacteriocin (1,280 AU/mL) of MLK27 strain strongly inhibited viable cell counts of L. monocytogenes adhered to HT-29 cells. However, there was no inhibitory effect of pathogen adhesion by bacteriocin treated with protease and inhibition activity of pathogen adhesion was partially decreased by heat-treated bacteriocin. According to biochemical and phylogenetic analysis, MLK27 strain was identified as Lactobacillus brevis MLK27. In conclusion, our results have shown that L. brevis MLK27 has ability to establish in human gastrointestinal tract and to prevent pathogenic L. monocytogenes infections, which is able to be considered as probiotic strains.