Spatial distribution and biodiversity of macrofauna in the Gorgan Bay, southeast of the Caspian Sea, were studied at fifteen stations in June 2010. Also, depth, temperature, salinity, dissolved oxygen, total organic matter content and sediment particle size were measured in each station. A total 3,356 individuals belonged to eight families and ten species were identified. Polychaeta were numerically dominated groups and Streblospio gynobranchiata, was constant and dominant species with 60.28% of total individuals but Bivalvia with four species had highest species number, though the density of them were low. The maximum density (4,500 ind/$m^2$) was obtained at station 1 while the minimum (411 ind/$m^2$) was observed at station 6. There was not significant correlation between the density of macrofauna with all environmental conditions. In total, six feeding group were considered but surface deposit feeder and deposit feeder were dominant in all stations. The maximum mean species number, diversity, richness, and evenness were obtained, 6.33, 1.46, 1.38 and 0.87, respectively. Based on the M-AMBI and the AMBI classification it seems that bentic environment in Gorgan Bay was not bad but the results of Shannon-Wiener, Margalef and Simpson indices the results were vice versa. In general, the values of the mentioned indices decreased from the western to the eastern part of the bay. Furthermore, the nonmetric multidimensional scaling (nMDS) showed that the structure of the macrofaunal assemblages was divides to six groups.