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In vitro Evaluation of Simvastatin Acid (SVA) Coated Beta-Tricalcium Phosphate (${eta}$-TCP) Particle on Bone Tissue Regeneration
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  • In vitro Evaluation of Simvastatin Acid (SVA) Coated Beta-Tricalcium Phosphate (${eta}$-TCP) Particle on Bone Tissue Regeneration
  • In vitro Evaluation of Simvastatin Acid (SVA) Coated Beta-Tricalcium Phosphate (${eta}$-TCP) Particle on Bone Tissue Regeneration
저자명
Yang. Dae-Hyeok,Bae. Min-Soo,Qiao. Lingjuan,Heo. Dong-Nyoung,Lee. Jung-Bok,Lee. Won-Jun,Park. Jae-Hong,Lee. Deok-Won,Hwang. Yu-S
간행물명
Macromolecular research
권/호정보
2012년|20권 7호|pp.754-761 (8 pages)
발행정보
한국고분자학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

The goal of this study was to evaluate the potential of beta-tricalcium phosphate (${eta}$-TCP) particles coated with difference concentrations (1 and 10 mM) of simvastatin acid (SVA) on bone formation in vitro. Changes in the surface morphologies and chemical compositions of SVA1-${eta}$-TCP and SVA10-${eta}$-TCP suggested that SVA was coated on their surface. These particles were further investigated by scanning electron microscopy (SEM) observations and X-ray photoelectron spectroscopy (XPS) measurements. By measuring ultraviolet/visible (UV/Vis) spectroscopy, we found that simvastatin acid (SVA) released in a sustained manner over the period of 28 days even though the initial burst happened within 1 day. These results verify that SVA1-${eta}$-TCP and SVA10-${eta}$-TCP can be useful as a biocompatible bone graft substitutes. Biocompatibility was evaluated by cytotoxicity tests, live/dead assay, and proliferation of preosteoblast cell-line (MC3T3-E1) cells culture. The results of assays for ALP activity, calcium deposition, and mRNA expressions of alkaline phosphatase (ALP) and osteopontin suggest that the amount of SVA plays an important role in accelerating bone formation in vitro.