Recently, interest in wooden construction have been growing by increasing needs and demands for eco-friendly and traditional wooden building(Hanok). Especially, Hanok has the technical development in manufacturing the mortise-tenon joint without fasteners(precut), so it could be called to modernization, industrialization and popularization. But the structural design and analysis of the structure were not regulated and had the difficulty to consider the variation of wooden member and to conduct the difficulty in the structural analysis and the design of the joint. In this study, the stiffness ratio of wooden mortise and tenon joint was evaluated according to the vertical loading, lintel and loading speed. The joint was distinguished in semi-rigid joint regardless of their factors. The stiffness ratio was 0.40 in vertical loading, 0.50 without vertical loading and 0.44 in horizontal loading with high speed. This study would be utilized to the structural analysis and design with structural analysis and design program.