In this paper, a setting method of initial cluster centers and color image segmentation using superpixels and Fuzzy C-means(FCM) algorithm is proposed. Generally, the FCM can be widely used to segment color images, and an element is assigned to any cluster with each membership values in the FCM. However the algorithm has a problem of local convergence by determining the initial cluster centers. So the selection of initial cluster centers is very important, we proposed an effective method to determine the initial cluster centers using superpixels. The superpixels can be obtained by grouping of some pixels having similar characteristics from original image, and it is projected $La^*b^*$ feature space to obtain the initial cluster centers. The proposed method can be speeded up because number of superpixels are extremely smaller than pixels of original image. To evaluate the proposed method, several color images are used for computer simulation, and we know that the proposed method is superior to the conventional algorithm by the experimental results.