The purpose of this study was to determine how smartphone use posture affects biomechanical variables and muscle activities. Eleven university students(age: $22.2{pm}2.6$ yrs, height: $176.6{pm}4.7$ cm, weight: $69.5{pm}7.5$ kg) who have no musculoskeletal disorder were recruited as the subject according to having experience in using the smartphone for more than one year. Angular velocity, muscle activity, and thumb finger pressure were determined for each trial. For each dependent variable, a one-way analysis of variance (ANOVA) with repeated measures was performed to test if significant difference existed among different three conditions (p<.05). The result showed that rotational angular velocity of the first metacarpal were increased in DESK posture compared with STAND posture during SU phase. The average nEMG values of FDI(First dorsal interosseous) were less in SIT and DESK posture compared with STAND posture during SR phase. These indicated that smartphone postures may effect the thumb ROM(Range of motion) and muscle activity. This has led to suggestions of the need for further kinetic and EMG analyses to evaluate best assess and characterize with smartphone use.