We initiate the study of interval-valued fuzzy quasi-ideal of a semigroup. In Section 2, we list some basic definitions in the later sections. In Section 3, we investigate interval-valued fuzzy subsemigroups and in Section 4, we define interval-valued fuzzy quasi-ideals and establish some of their basic properties. In Section 5, we obtain characterizations of regular and intraregular semigroups using the machinery developed in the preceding sections.