There has been increasing number of patients with asthma, rhinitis, and conjunctivitis due to the atmospheric pollution and global warming. In this study, we investigated daily observed number of pollens to establish a standardized method for the impact analysis of the climate changes on the number of daily pollens in Korea. Daily observed allergenic pollens of trees, weeds, and pine were analyzed during the period of 1998 to 2010 (except 2006) in Seoul, Busan, Daegu, Gwangju, Gangneung, and Jeju. They increased in large cities including Seoul during the last 12 years and the long-term trend may continue in the future. Daily concentration or amount of pollens was highly correlated with weather variables. Positive correlation was found between air temperature and the pollens although different relationships existed for different locations. Chill days were utilized to estimate the length of the flowering or pollen period. The pollen period of trees was shorter in the field when the winter temperature was low. This approach may be utilized to determine the quantitative change in length of the pollen season in the future.