The purpose of the study were to investigate the effect of the neuro-feedback program which improves brain function and stress reduction effect. The research design is one group pretest-posttest survey. 121 nursing students of a college in the Busan region took part in the study. Training involving the neuro-feedback program was conducted twice a week, 30 minutes per session, for a total of 10 weeks. The collected data was encoded and analyzed using SPSS 12.0 Version. The brain function and stress levels of the subjects were analyzed through the before-and-after results of the training were analyzed using a paired t-test. The results of the study showed that the BQ and SQ were enhanced as a result of the neuro-feedback. In particular, there were significant increases in the SRQ, ATQ, ACQ, and EQ of the BQ. SQ is correlated with the prevalence rate and resistance to disease, meaning not only psychological anxiety, uneasiness and excitement, but also physical anxiety and response to disease. Based on the study, by using the neuro-feedback training as a program for stress reduction, it is expected that nursing students will receive less stress from internal and external factors, and their ability to cope with stress will be enhanced.