다양한 화장품에 응용을 위하여 제주산 유채오일 추출물을 대상으로 PIT유화시스템을 이용한 나노에멀젼을 제조하였다. 천연 유채오일 추출물은 n-헥산을 용매로 사용하여 추출하였다. 천연유채오일 추출물은 엷은 노란색의 점성을 가진 액체이었고, 수율은 $43{pm}2.5%$이었다. 산가는 $2.76{pm}0.5$이었고, 비중은 $0.89{pm}0.05$ 이었다. 20wt%의 유채오일을 사용한 PIT-Yuche-NE의 입자크기는 50-120nm (평균입자크기: $82{pm}5.8nm$)이었고, 제타 포텐셜은 -29.5mV 이었다. 이것은 $(PEG)_{5-30}$ 지방산 에테르를 사용하기 때문에 열역학적으로 안정하였다. 특징적인 결과로부터 얻은 몇 개의 결론을 다음과 같이 나열하였다. 첫째, DPPH (1,1-diphenyl-2-picrylhydrazyl radical)방법을 이용한 자유 라디칼 소거력을 측정하였다. PIT-Yuche-NE의 항산화력은 $37.2{pm}6.7%$이었고, 비교군인 10 mg/mL의 PIT-Toco-NE(토코페롤 20wt% 나노에멀젼)은 $28.8{pm}6.5%$, 10 mg/mL의 PIT-Nokcha-NE (녹차추출물 20wt% 나노에멀젼)은 $29.6{pm}7.2%$이었다. 둘째, PIT-Yuche-NE의 콜라겐합성율은 $148{pm}15.2%$ 이었고, 동일 농도에서 비교군인 PIT-Toco-NE은 $121{pm}13.5%$, PIT-Nokcha-NE은 $95{pm}12.7%$이었다. 셋째, 6시간 후, Aramo-TS를 사용한 Yuche-CRM의 피부보습효과는 $47{pm}3.9%$ (*p-value￡0.05, n=7)이었다. 반면, Toco-CRM은 $30{pm}5.2%$(*p-value￡0.05, n=7)이었고, Nokcha-CRM은 $35{pm}4.5%$이었다. 따라서 Yuche-CRM은 다른 두 크림보다 높은 보습효과를 보였다. 최종적으로 본 연구는 화장품 산업 및 제약산업에서도 폭넓게 응용될 것으로 기대한다.
Nano-emulsion with phase inversion temperature (PIT) emulsifying system was prepared to use rapeseed oil from originating Jeju in order to apply various cosmetic applications. Natural rape seed oil (NRSO) extraction was extracted using n-hexane as a solvent. NRSO extract showed a light yellowish color of viscous liquid as well as yield was $43{pm}2.5%$. Acid value was $2.76{pm}0.5$ and gravity was $0.89{pm}0.05$. Droplet size of PIT-Yuche-NE with 20wt% of rapeseed oil was 50-120nm (average: $82{pm}5.8nm$) and zeta potential was -29.5mV. It was thermodynamically good stable emulsion due to $(PEG)_{5-30}$fattyacidether. Some conclusions from the result of characteristic experiment were obtained as follows. First, the anti-oxidative activity was measured by free radical scavenging activity using DPPH (1,1-diphenyl-2-picrylhydrazyl radical). Anti-oxidative activity of PIT-Yuche-NE was $37.2{pm}6.7%$ on 10mg/mL compared with PIT-Toco-NE (Natural tocopherol nano-emulsion, $28.8{pm}6.5%$ on 10 mg/mL) and PIT-Nokcha-NE (Green tea extract nano-emulsion, $29.6{pm}7.2%$ on 10mg/mL). Second, the collagen synthesis activity of PIT-Yuche-NE was $148{pm}15.2%$ compared with PIT-Toco-NE (Natural tocopherol nano-emulsion, $121{pm}13.5%$ on 10mg/mL) and PIT-Nokcha-NE (Green tea extract nano-emulsion, $95{pm}12.7%$ on 10mg/mL). Third, the effectiveness of moisturizing activity of Yuche-CRM with Aramo-TS after 6 hours increase $47{pm}3.9%$ (*p-value￡0.05, n=7) whereas Both Toco-CRM was $30{pm}5.2%$ (*p-value￡0.05, n=7) and Nokcha-CRM was $35{pm}4.5%$. Therefore, Yuche-CRM has higher moisturizing effect than other two creams. Finally, Nano-emulsion stabilizing rapeseed oil using PIT emulsifying system of this study can be used to apply cosmetics industry and pharmaceutical industry.
