Instrumentation and control system in nuclear power plant performs protecting, controling and monitoring safety operation of Nuclear Power Plant. As cyber attack to the control equipment of instrumentation and control system can cause reactor shutdown and radiation release, it is required to design the instrumentation and control system considering cyber security in accordance with regulatory guides and industrial standards. In this paper, we proposed a design method of uni-directional communication structure which is required in the design of defense-in-depth model according to regulatory guides and industrial standards and we implemented a communication board with the proposed method. This communication board was tested in various test environments and test items and we concluded it can provide uni-directional communication structure required to design of defense-in-depth model against cyber attack by analyzing the results. The proposed method and implemented communication board were applied in the design of SMART (system-integrated modular advanced reactor) I&C (instrumentation and control) systems.