The hydration and antimicrobial peptide effects on the lipid alignment on the surface of a thin glass plate were investigated by using the solid-state nuclear magnetic resonance spectroscopy. Pure 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphotidylcholine (POPC) lipid molecules were well aligned on the surface of a thin glass plate without direct hydration by placing the sample for a few days in the desiccator containing a saturated sodium phosphate dibasic solution, which provided 95% relative humidity. But there was a big difference between two lipid phases, the one of which was hydrated by placing the sample for a few days in the desiccator with 95% relative humidity without direct hydration and the other was directly hydrated by dropping water and then placed for a few days in the same desiccator. The surface mobility of POPC molecules in a lipid bilayer phase was much activated by water. While the time for the POPC to align on the glass plate surface was short, the time for the PG-1/POPC mixture to reach the its equilibrium state was long.