Objectives : The purpose of this study was to investigate the level of expertise and awareness of information on toothpaste in dental hygienists and to give patients information on toothpaste. Methods : The subjects was 205 dental hygienists working at dental clinic, dental hospital or college dental hospital in Seoul and Gyeonggi-do. They filled out the questionaire from 13th to 27th April, 2013. Collected data were analyzed by SPSS 21.0 program and significant level was set at p=0.05. Results : The awareness of information on toothpaste was the highest in dental hygienists having under 3 years career and working at dental clinics(p<0.001). The level of expertise on toothpaste was the highest in those having over 5 years career and working at general hospital or college dental hospital(p<0.001). In association with the effect of toothpaste(p<0.05), experienced dental hygienists had a higher knowledge(1.68 point) that those who had no experience(2.27 point). Instruction of the oral care devices included 27.5% of dental floss, 27.1% of toothbrush and 25.4% of proxabrush, and 4.9% of toothpaste. Conclusions : The results revealed that the right choice of the toothpaste would be the best prevention of dental caries and it is necessary to educate the dental hygienists for the toothpaste information.