PURPOSE: This study was performed to investigate the effect of Low-dye Taping on muscle activity during single-leg standing in subjects with flexible flatfoot. METHODS: Thirteen able-body volunteers who had flexible flatfoot were recruited for this study. Subjects were measured navicular drop test to evaluate pronation of foot and muscle activity during single-leg standing before and after taping. The muscle activity was recorded using surface EMG from the tibialis anterior and the peroneus longus during single-leg standing on stable and unstable surface. RESULTS: The results show that the navicular drop height and the tibialis anterior muscle activity were significantly decreased after Low-dye taping. CONCLUSION: The results suggest that Low-dye taping could be useful in managing overuse of the tibialis anterior by reducing their level of activation during single-leg standing.