Recently student portfolio is widely used as an evaluation method for self-directed performance in engineering education of the university. Especially, as many of the Innovation Centers for Engineering Education and ABEEK held student portfolio competitions, a lot of students in engineering college participate in student portfolio includes the contents and careers of the entire process of university. However, except for courses related to capstone design, there are few regular courses applied to Learning Portfolio in engineering education. Through using Learning Portfolio in courses, students take opportunities of organized collection of learning contents and critical reflection. Under these background study, the regular course in engineering education is may be appropriate for using Learing Portfolio in lecture. In this paper, we present the case study for the Data Structure course opened in the first semester of 2013 which is organized as a Learning Portfolio. According to the results of applying Learning Portfolio to the regular course, it turns out that learners can develope self-directed learning ability in exploring the learning process, and manage a learning process systematically through self-reflection in learning process.