This study analyzes structural analysis with fatigue and natural frequency on ATV knuckle. The maximum equivalent stresses are happened at the end of knuckle in case of model 1, 2 and 3. As these stresses are below the allowable stress, these models can be stable structurally. The fatigue damage possibility at model 1 becomes more than model 2 and 3. Model 2 or 3 has more durability than model 1 at fatigue. As the resonances are happened at the frequency more than 2000 Hz in case of model 1, 2 and 3, there is no resonance possibilities at real driving. Prevention against damage and durability prediction on automotive chassis parts can be effectively improved by applying this study result on knuckle and improving structural strength.