In this study, a biological odor treatment system was proposed to remove odor(foul smell) materials causing several problems in the closed sewage treatment plant. This odor treatment system was composed of a two-step biofilter system in one reactor. The two-step biofilter reactor was constructed with natural purification layer in upper part and artificial purification layer in lower part. The reed grasses of water purification plants were planted in the surface area and mixed porous ceramic media were filled with the lower part of biofilter reactor. By using the above experimental apparatus, the ammonia gas removal efficiency was attained to 98.3 % and the hydrogen sulfide gas removal efficiency was appeared more than 97.7 % which shows more effective than the conventional odor removal process.