Objectives: To explore the relationships between age, cytogenetic subgroups, molecular markers, and cells with leukemic aberrant immunophenotype in patients with acute myeloid leukemia (AML). Methods: In this study, we evaluated the correlations between age, cytogenetic subgroups (normal, balanced and unbalance karyotype), molecular mutations (NPM1, FLT3-ITD, and CEBPA mutations) and marrow leukemia cells (LC) identified by flow cytometry in 256 patients with de novo AML. Results: From age group 10-19 years to age group ${geq}60$ years, the percentage of LC decreased from $67.0{pm}18.4%$ to $49.0{pm}25.1%$ (F=2.353, P=0.041). LC percentage was higher in patients with balanced karyotypes ($65.7{pm}22.4%$), than those with unbalanced karyotypes ($46.0{pm}26.6%$) (u=3.444, P=0.001) or a normal karyotype ($49.9{pm}22.1%$) (u=5.093, P<0.001). Patients with FLT3-ITD ($64.3{pm}19.5%$) had higher LC percentages compared with those without ($54.2{pm}24.3%$) (u=2.794, P=0.007). Conclusions: Associations between age, cytogenetics, molecular markers, and marrow leukemia cells may offer beneficial information to understand the biology and pathogenesis of AML.