홍마늘 추출물(RG)과 홍마늘 추출물에 녹차 및 식이섬유를 혼합한 복합물(RGT, RGF 및 RGTF)이 고지방-콜레스테롤 식이성 흰쥐에 급이 시 체내 지질 배설 및 간 조직의 항산화 효소활성에 미치는 영향을 알아보고 in vitro에서 이들 복합물의 항산화 활성을 비교하였다. in vitro에서 DPPH 라디칼 소거활성은 홍마늘 추출물(RG)에 비해 RGT 및 RGTF에서 높았으며, ABTs 라디칼 소거활성은 RGT와 RGTF의 활성이 유사하였고 이는 RG보다 유의적으로 높은 활성이었다. 환원력은 라디칼 소거활성과 비슷한 경향이었다. 간 조직의 총 지질, 심장 조직의 중성지방 함량은 HRG군에 비해 홍마늘 복합물 급이군에서 유의적인 감소를 보였다. 실험 식이를 급이한 4주 후에 분변 중의 총 지질 함량은 HRGF 및 HRGTF군이 대조군에 비해 유의적으로 증가되었다. 간 조직의 지질과산화물 함량은 대조군에 비해 홍마늘 복합물 급이군에서 6.2~12.1% 감소되었으며, 항산화 활성은 복합물 급이군이 대조군에 비해 유의적으로 증가되었으나 그 차이는 미미하였다. 간 조직의 SOD 활성은 복합물 급이군이 HRG군보다 높았다. Catalase 활성은 HRGT군, GSH-px 활성은 HRGT 및 HRGTF군의 활성이 대조군보다 높았다. UDPGT 활성은 대조군에 비해 HRGT 및 HRGTF군에서 유의적으로 높았다. 따라서 홍마늘 복합물은 시료 중의 페놀 화합물, 식이섬유에 의해 분변 중으로 지질 배설작용이 촉진됨으로써 간 조직의 지질 수준 감소와 항산화 효소활성의 증가에 기여하는 것으로 생각된다.
This study was performed to investigate the effects of the hot water extract of red garlic (RG) and RG-composites on fecal lipid levels and hepatic antioxidant enzyme activity in rats fed a high fat-cholesterol diet. Three different types of RG-composites prepared: RG and green tea (RGT), RG and dietary fiber (RGF), and RG, green tea, and dietary fiber (RGTF). Rats were divided into six groups: the control, the group fed a high fat-cholesterol diet (HFC), the RG-supplemented group (HRG), the RGT supplemented group (HRGT), the RGF supplemented group (HRGF), and the RGTF supplemented with HFC group (HRGTF). The antioxidant activity of these composites was tested, in vitro. The DPPH radical scavenging activity was higher RGT and RGTF than RG. ABTs radical scavenging activity of RGT was similar to RGTF. Their activities were significantly higher than RG. The reducing power was similar to their radical scavenging activities. Total lipid levels in the liver and triglyceride levels in the heart were lower in the groups fed RG-composites than the HRG group. Fecal total lipid level was higher in the HRGF and HRGTF groups than the HRG group after 4 weeks diet supplementation during 4 weeks. Lipid peroxide content was reduced to anywhere between 6.2% and 12.1% in the groups fed RG-composites, compared to the HFC group. Antioxidant activity was significantly higher in the groups fed RG-composites than the HFC group. Hepatic SOD activity was higher in the groups fed RG-composites than the HRG group. The HRGT group in catalase activity, and the HRGT and HRGTF groups in GSH-px activity were increased significantly compared to the HFC group. Hepatic UDPGT activity was increased significantly in the HRGT and HRGTF groups to the HFC group, as well. These results indicate that antioxidant activities of the RG-composites were related to the decrease of lipid levels by increasing the fecal excretion and enhancement of hepatic antioxidant enzyme activity in rats fed a high fat-cholesterol diet.
