This paper presents the response calibration method based on quality control range, which is established from the concept and method of statistical quality control for natural frequency ratio and response ratio. To this end, statistical analysis including descriptive statistics analysis, normality test, ANOVA were performed for response characteristics obtained from loading tests and structural analysis for more than hundred and thirty well-conditioned bridges. Suggested method is based on real structural integrity evaluation case studies and statistical quality control approach, in this respect it is expected to provide scientific criteria and systematic procedure for response calibration and load carrying capacity assessment.