In this paper, we propose a multi-band monopole antenna with a band-notching U-slot, which is fabricated inside the flexible polymide film substrate. The U-shaped slot located on the patch-shaped monopole antenna provides band-notch at 2.7 GHz, but also helps to improve return loss at adjacent frequency bands. The performance of the antenna attached on an automobile-glass has been simulated and measured. The fabricated antenna provides more than 10 dB return loss for ISM band(2.4~2.483 GHz) and WAVE band(5.85~5.925 GHz), 2.8~5.7 dBi maximum gain, and good radiation patterns.