It is possible to obtain an omnidirectional stereo image via a single camera by using a catadioptric approach with a convex mirror and concave lens. In order to measure three-dimensional distance using the imaging system, the optical parameters of the system are required. In this paper, a calibration procedure to extract the parameters of the imaging system is described. Based on the parameters, experiments are carried out to verify the performance of the three-dimensional distance measurement of a single camera omnidirectional stereo imaging system.