This study was done to investigate and compare satisfaction of clinical practice and its influencing factors between associate and bachelor nursing students. The participants were 420 nursing students in two cities. Data were collected through self-report questionnaires, and analyzed using the SPSS/WIN 19.0 program. Satisfaction of clinical practice in associate and bachelor nursing students correlated with self-esteem, self-efficacy, interpersonal relationships, and satisfaction of major. Significant factor influencing satisfaction of clinical practice in associate nursing students was satisfaction of major, and this factor accounted for 26.8% of the variance in satisfaction of clinical practice. And significant factors influencing satisfaction of clinical practice in bachelor nursing students were satisfaction of major, interpersonal relationships and gender, and these factors accounted for 31.9% of the variance in satisfaction of clinical practice. The above findings indicate that satisfaction of clinical practice in nursing students is influenced by satisfaction of major and interpersonal relationships. Therefore, when nursing interventions are developed to improve satisfaction of clinical practice in nursing students, these factors need to be considered.