Objectives: In working environment measurement, sampling is an important stage for obtaining reliable result as analysis. A personal air sampling pump is one of the most fundamental and important element in the work environment measurement, but it remains at the level of calibrating the flow rate of the pump before and after sampling. There is no checking whether the flow rate set at the initial stage would be hold during sampling. The purpose of this study was to develop a method to evaluate the flow rate performance of particulate sampling pump with three-pieces cassette holder containing filters commonly used to sample particulate. Materials and methods: We tested back pressure of particulate sampling pumps commonly used in Korea with three-pieces cassette holder containing various filters, and tried to find out the combination conditions of filters in accordance with back pressure required by ISO standard 13137. Results: We found out the matrix of sampling media such as three-pieces cassette holder containing filters applicable to the pressure drop required by the ISO standard for evaluating the flow rate stability under increasing pressure drop and long term(8 hour) performance. Conclusions: This evaluation method using sampling media matrix for checking flow rate stability proposed by this study could be very useful tool to find out good performance pumps before sampling.