Recently a novel cycle named organic flash cycle (OFC) has been proposed which has improved potential for power generation from low-temperature heat sources. This study carries out thermodynamic performance analysis of OFC using various working fluids for recovery of low-grade heat sources in the form of sensible energy. Special attention is focused on the optimum flash temperature at which the exergy efficiency has the maximum value. Under the optimal conditions with respect to the flash temperature, the thermodynamic performances of important system variables including mass flow ratio, separation ratio, heat addition, specific volume flow rate at turbine exit, and exergy efficiency are thoroughly investigated. Results show that the exergy efficiency has a peak value with respect to the flash temperature and the optimum working fluid which shows the best exergy efficiency varies with the operating conditions.